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cre recombinase  (Addgene inc)


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    Addgene inc cre recombinase
    Cre Recombinase, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 354 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cre+recombinase/CRE+recombinase+(Plasmid+%2362730)/pm41928626-197-24-34
    Average 96 stars, based on 354 article reviews
    cre recombinase - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: eSPLIT and iSWAP: CRISPR-Mediated Conditional Allele Engineering with Short Artificial Introns
    Article Snippet: Sequencing data was analyzed using SnapGene® software (Dotmatics). .. Lentivirus expressing Cre recombinase was produced by co-transfecting HEK293T cells with plasmids encoding the Cre recombinase and mCherry under the control of CMV promoter (pLenti-mCherry-Cre-blast (Addgene#179390)) together with psPAX2 (Addgene #12260) and pMD2.G (addgene #12259) which encode Gag, Pol, Rev proteins for lentiviral packaging and VSV-G envelope respectively. ..

    Article Title: Selenoprotein H Functions as a PPARα Coactivator to Link Selenium Homeostasis to Hepatic Lipid Metabolism and Protect against Steatohepatitis.
    Article Snippet: For plasmids used in AAV packaging, the liver-specific CRISPR AAV vector, AAV-TBG-Cre-luciferase-sgRNA, used in this study was constructed by modifying the pAAV-GFP plasmid (Cell Biolabs). .. The original CMV promoter was replaced with the liver-specific TBG promoter, and the original GFP cassette was replaced with the Cre-T2A-luciferase-sgRNA cassette for expression of Cre recombinase, luciferase and sgRNA, in which the sgRNA scaffold was derived from the lentiCRISPR-V2 plasmid (Addgene, 52961). ..

    Produced:

    Article Title: eSPLIT and iSWAP: CRISPR-Mediated Conditional Allele Engineering with Short Artificial Introns
    Article Snippet: Sequencing data was analyzed using SnapGene® software (Dotmatics). .. Lentivirus expressing Cre recombinase was produced by co-transfecting HEK293T cells with plasmids encoding the Cre recombinase and mCherry under the control of CMV promoter (pLenti-mCherry-Cre-blast (Addgene#179390)) together with psPAX2 (Addgene #12260) and pMD2.G (addgene #12259) which encode Gag, Pol, Rev proteins for lentiviral packaging and VSV-G envelope respectively. ..

    Control:

    Article Title: eSPLIT and iSWAP: CRISPR-Mediated Conditional Allele Engineering with Short Artificial Introns
    Article Snippet: Sequencing data was analyzed using SnapGene® software (Dotmatics). .. Lentivirus expressing Cre recombinase was produced by co-transfecting HEK293T cells with plasmids encoding the Cre recombinase and mCherry under the control of CMV promoter (pLenti-mCherry-Cre-blast (Addgene#179390)) together with psPAX2 (Addgene #12260) and pMD2.G (addgene #12259) which encode Gag, Pol, Rev proteins for lentiviral packaging and VSV-G envelope respectively. ..

    Recombinant:

    Article Title: Medial prefrontal cortex activity precedes dorsomedial striatum in need for change during history-based flexible behavior
    Article Snippet: .. Retrograde recombinant adeno-associated virus (AAV) containing Cre recombinase (pAAV-Ef1ɑ-mCherry-IRES-Cre, a gift from Karl Deisseroth, Addgene plasmid # 55632; http://n2t.net/addgene:55632 ; RRID: Addgene_55632), was obtained from Addgene (Addgene viral prep # 55632-AAVrg). .. AAV serotype 1 containing ChR2 (H134R) (pAAV-Ef1ɑ-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA, a gift from Karl Deisseroth, Addgene plasmid # 20298; http://n2t.net/addgene:20298 ; RRID: Addgene_20298), was obtained from Addgene (Addgene viral prep # 20298-AAV1).

    Virus:

    Article Title: Medial prefrontal cortex activity precedes dorsomedial striatum in need for change during history-based flexible behavior
    Article Snippet: .. Retrograde recombinant adeno-associated virus (AAV) containing Cre recombinase (pAAV-Ef1ɑ-mCherry-IRES-Cre, a gift from Karl Deisseroth, Addgene plasmid # 55632; http://n2t.net/addgene:55632 ; RRID: Addgene_55632), was obtained from Addgene (Addgene viral prep # 55632-AAVrg). .. AAV serotype 1 containing ChR2 (H134R) (pAAV-Ef1ɑ-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA, a gift from Karl Deisseroth, Addgene plasmid # 20298; http://n2t.net/addgene:20298 ; RRID: Addgene_20298), was obtained from Addgene (Addgene viral prep # 20298-AAV1).

    Article Title: Bioengineering mini-colons for ex vivo colorectal cancer research.
    Article Snippet: Tumor initiation remains one of the least understood events in cancer biology, largely due to the challenge of dissecting the intricacy of the tumorigenic process in laboratory settings.. The insufficient biological complexity of conventional in vitro systems makes animal models the primary experimental approach to study tumorigenesis.. Despite providing valuable insights, these in vivo models function as experimental black boxes with limited spatiotemporal resolution of cellular dynamics during oncogenesis.

    Article Title: Co-repression of Yap1 and Sox9 Abrogates Established Cholangiocarcinoma by Eliminating Transcriptional Compensation.
    Article Snippet: For pX330-sgP19 (sgp19), pX330 (Addgene #42230) was used as the template, with a single sgp19 duplex integrated. sgRNA sequences for the target genes are listed in Supplementary Table 10. .. AAV8-TBG/Cre and Tamoxifen administration Mice were injected intravenously with 1 × 1012 genome copies (GCs) of adeno-associated virus serotype 8 (AAV8) encoding eGFP or Cre recombinase under the hepatocyte-specific thyroid binding globulin promoter (AAV8-TBG-eGFP; Addgene#105535-AAV8, AAV8-TBG-Cre; Addgene#107787-AAV8) in 100 ul saline. .. CreERT2 recombinase was induced by intraperitoneal tamoxifen injections (20 mg/ml) in corn oil (Thermo Scientific#AC405435000) as previously described elsewhere(5).

    Bioprocessing:

    Article Title: Medial prefrontal cortex activity precedes dorsomedial striatum in need for change during history-based flexible behavior
    Article Snippet: .. Retrograde recombinant adeno-associated virus (AAV) containing Cre recombinase (pAAV-Ef1ɑ-mCherry-IRES-Cre, a gift from Karl Deisseroth, Addgene plasmid # 55632; http://n2t.net/addgene:55632 ; RRID: Addgene_55632), was obtained from Addgene (Addgene viral prep # 55632-AAVrg). .. AAV serotype 1 containing ChR2 (H134R) (pAAV-Ef1ɑ-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA, a gift from Karl Deisseroth, Addgene plasmid # 20298; http://n2t.net/addgene:20298 ; RRID: Addgene_20298), was obtained from Addgene (Addgene viral prep # 20298-AAV1).

    Article Title: A prodrug targeting CIM6P/IGF2R enhances memory in healthy mice and reverses deficits in an Angelman syndrome mouse model.
    Article Snippet: .. Cloning of Cre recombinase into plasmid and AAV production Coding sequences for NLS-T2A with or without Cre recombinase were amplified from LFNG:GFPnls-T2A-ER-Cre-ER (AddGene, #200107, AddGene) by PCR using Q5 HighFidelity 2x Master Mix (M0492S, NEB, Ipswich, MA). .. The two amplified fragments were cloned into the BamHI site of pAAVSyn-GFP (AddGene #58867) using HiFi Assembly 2xMaster Mix (NEB, E2621S) to generate pAAV-Syn-Cre-NLS-T2A-eGFP.

    Plasmid Preparation:

    Article Title: Medial prefrontal cortex activity precedes dorsomedial striatum in need for change during history-based flexible behavior
    Article Snippet: .. Retrograde recombinant adeno-associated virus (AAV) containing Cre recombinase (pAAV-Ef1ɑ-mCherry-IRES-Cre, a gift from Karl Deisseroth, Addgene plasmid # 55632; http://n2t.net/addgene:55632 ; RRID: Addgene_55632), was obtained from Addgene (Addgene viral prep # 55632-AAVrg). .. AAV serotype 1 containing ChR2 (H134R) (pAAV-Ef1ɑ-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA, a gift from Karl Deisseroth, Addgene plasmid # 20298; http://n2t.net/addgene:20298 ; RRID: Addgene_20298), was obtained from Addgene (Addgene viral prep # 20298-AAV1).

    Article Title: Selenoprotein H Functions as a PPARα Coactivator to Link Selenium Homeostasis to Hepatic Lipid Metabolism and Protect against Steatohepatitis.
    Article Snippet: For plasmids used in AAV packaging, the liver-specific CRISPR AAV vector, AAV-TBG-Cre-luciferase-sgRNA, used in this study was constructed by modifying the pAAV-GFP plasmid (Cell Biolabs). .. The original CMV promoter was replaced with the liver-specific TBG promoter, and the original GFP cassette was replaced with the Cre-T2A-luciferase-sgRNA cassette for expression of Cre recombinase, luciferase and sgRNA, in which the sgRNA scaffold was derived from the lentiCRISPR-V2 plasmid (Addgene, 52961). ..

    Article Title: A prodrug targeting CIM6P/IGF2R enhances memory in healthy mice and reverses deficits in an Angelman syndrome mouse model.
    Article Snippet: .. Cloning of Cre recombinase into plasmid and AAV production Coding sequences for NLS-T2A with or without Cre recombinase were amplified from LFNG:GFPnls-T2A-ER-Cre-ER (AddGene, #200107, AddGene) by PCR using Q5 HighFidelity 2x Master Mix (M0492S, NEB, Ipswich, MA). .. The two amplified fragments were cloned into the BamHI site of pAAVSyn-GFP (AddGene #58867) using HiFi Assembly 2xMaster Mix (NEB, E2621S) to generate pAAV-Syn-Cre-NLS-T2A-eGFP.

    Article Title: ARF-1 Coordinates Cargo Sorting at FERARI Endosomal Recycling Hubs
    Article Snippet: .. Afterwards to excise the loxP-flanked G418 antibiotic resistance cassette, cells were transfected with a Cre-recombinase (addgene plasmid #11923) using FuGENE. ..

    Luciferase:

    Article Title: Selenoprotein H Functions as a PPARα Coactivator to Link Selenium Homeostasis to Hepatic Lipid Metabolism and Protect against Steatohepatitis.
    Article Snippet: For plasmids used in AAV packaging, the liver-specific CRISPR AAV vector, AAV-TBG-Cre-luciferase-sgRNA, used in this study was constructed by modifying the pAAV-GFP plasmid (Cell Biolabs). .. The original CMV promoter was replaced with the liver-specific TBG promoter, and the original GFP cassette was replaced with the Cre-T2A-luciferase-sgRNA cassette for expression of Cre recombinase, luciferase and sgRNA, in which the sgRNA scaffold was derived from the lentiCRISPR-V2 plasmid (Addgene, 52961). ..

    Derivative Assay:

    Article Title: Selenoprotein H Functions as a PPARα Coactivator to Link Selenium Homeostasis to Hepatic Lipid Metabolism and Protect against Steatohepatitis.
    Article Snippet: For plasmids used in AAV packaging, the liver-specific CRISPR AAV vector, AAV-TBG-Cre-luciferase-sgRNA, used in this study was constructed by modifying the pAAV-GFP plasmid (Cell Biolabs). .. The original CMV promoter was replaced with the liver-specific TBG promoter, and the original GFP cassette was replaced with the Cre-T2A-luciferase-sgRNA cassette for expression of Cre recombinase, luciferase and sgRNA, in which the sgRNA scaffold was derived from the lentiCRISPR-V2 plasmid (Addgene, 52961). ..

    Transduction:

    Article Title: Oligodendrocyte-derived extracellular vesicles for therapy of multiple sclerosis
    Article Snippet: Human OPCs derived from NIH-approved H9 human ESCs (Millipore) were grown for three weeks and differentiated into mature Ols, according to Millipore protocol. .. Cell Transduction OPCs and HEK cells were transduced with a lentivirus coding for Cre recombinase (Lv-Cre; Addgene #12106), or mouse MOG (Lv-MOG, Origene). ..

    Cloning:

    Article Title: A prodrug targeting CIM6P/IGF2R enhances memory in healthy mice and reverses deficits in an Angelman syndrome mouse model.
    Article Snippet: .. Cloning of Cre recombinase into plasmid and AAV production Coding sequences for NLS-T2A with or without Cre recombinase were amplified from LFNG:GFPnls-T2A-ER-Cre-ER (AddGene, #200107, AddGene) by PCR using Q5 HighFidelity 2x Master Mix (M0492S, NEB, Ipswich, MA). .. The two amplified fragments were cloned into the BamHI site of pAAVSyn-GFP (AddGene #58867) using HiFi Assembly 2xMaster Mix (NEB, E2621S) to generate pAAV-Syn-Cre-NLS-T2A-eGFP.

    Amplification:

    Article Title: A prodrug targeting CIM6P/IGF2R enhances memory in healthy mice and reverses deficits in an Angelman syndrome mouse model.
    Article Snippet: .. Cloning of Cre recombinase into plasmid and AAV production Coding sequences for NLS-T2A with or without Cre recombinase were amplified from LFNG:GFPnls-T2A-ER-Cre-ER (AddGene, #200107, AddGene) by PCR using Q5 HighFidelity 2x Master Mix (M0492S, NEB, Ipswich, MA). .. The two amplified fragments were cloned into the BamHI site of pAAVSyn-GFP (AddGene #58867) using HiFi Assembly 2xMaster Mix (NEB, E2621S) to generate pAAV-Syn-Cre-NLS-T2A-eGFP.

    Polymerase Chain Reaction:

    Article Title: A prodrug targeting CIM6P/IGF2R enhances memory in healthy mice and reverses deficits in an Angelman syndrome mouse model.
    Article Snippet: .. Cloning of Cre recombinase into plasmid and AAV production Coding sequences for NLS-T2A with or without Cre recombinase were amplified from LFNG:GFPnls-T2A-ER-Cre-ER (AddGene, #200107, AddGene) by PCR using Q5 HighFidelity 2x Master Mix (M0492S, NEB, Ipswich, MA). .. The two amplified fragments were cloned into the BamHI site of pAAVSyn-GFP (AddGene #58867) using HiFi Assembly 2xMaster Mix (NEB, E2621S) to generate pAAV-Syn-Cre-NLS-T2A-eGFP.

    Transfection:

    Article Title: ARF-1 Coordinates Cargo Sorting at FERARI Endosomal Recycling Hubs
    Article Snippet: .. Afterwards to excise the loxP-flanked G418 antibiotic resistance cassette, cells were transfected with a Cre-recombinase (addgene plasmid #11923) using FuGENE. ..

    Mouse Assay:

    Article Title: Co-repression of Yap1 and Sox9 Abrogates Established Cholangiocarcinoma by Eliminating Transcriptional Compensation.
    Article Snippet: For pX330-sgP19 (sgp19), pX330 (Addgene #42230) was used as the template, with a single sgp19 duplex integrated. sgRNA sequences for the target genes are listed in Supplementary Table 10. .. AAV8-TBG/Cre and Tamoxifen administration Mice were injected intravenously with 1 × 1012 genome copies (GCs) of adeno-associated virus serotype 8 (AAV8) encoding eGFP or Cre recombinase under the hepatocyte-specific thyroid binding globulin promoter (AAV8-TBG-eGFP; Addgene#105535-AAV8, AAV8-TBG-Cre; Addgene#107787-AAV8) in 100 ul saline. .. CreERT2 recombinase was induced by intraperitoneal tamoxifen injections (20 mg/ml) in corn oil (Thermo Scientific#AC405435000) as previously described elsewhere(5).

    Injection:

    Article Title: Co-repression of Yap1 and Sox9 Abrogates Established Cholangiocarcinoma by Eliminating Transcriptional Compensation.
    Article Snippet: For pX330-sgP19 (sgp19), pX330 (Addgene #42230) was used as the template, with a single sgp19 duplex integrated. sgRNA sequences for the target genes are listed in Supplementary Table 10. .. AAV8-TBG/Cre and Tamoxifen administration Mice were injected intravenously with 1 × 1012 genome copies (GCs) of adeno-associated virus serotype 8 (AAV8) encoding eGFP or Cre recombinase under the hepatocyte-specific thyroid binding globulin promoter (AAV8-TBG-eGFP; Addgene#105535-AAV8, AAV8-TBG-Cre; Addgene#107787-AAV8) in 100 ul saline. .. CreERT2 recombinase was induced by intraperitoneal tamoxifen injections (20 mg/ml) in corn oil (Thermo Scientific#AC405435000) as previously described elsewhere(5).

    Binding Assay:

    Article Title: Co-repression of Yap1 and Sox9 Abrogates Established Cholangiocarcinoma by Eliminating Transcriptional Compensation.
    Article Snippet: For pX330-sgP19 (sgp19), pX330 (Addgene #42230) was used as the template, with a single sgp19 duplex integrated. sgRNA sequences for the target genes are listed in Supplementary Table 10. .. AAV8-TBG/Cre and Tamoxifen administration Mice were injected intravenously with 1 × 1012 genome copies (GCs) of adeno-associated virus serotype 8 (AAV8) encoding eGFP or Cre recombinase under the hepatocyte-specific thyroid binding globulin promoter (AAV8-TBG-eGFP; Addgene#105535-AAV8, AAV8-TBG-Cre; Addgene#107787-AAV8) in 100 ul saline. .. CreERT2 recombinase was induced by intraperitoneal tamoxifen injections (20 mg/ml) in corn oil (Thermo Scientific#AC405435000) as previously described elsewhere(5).

    Saline:

    Article Title: Co-repression of Yap1 and Sox9 Abrogates Established Cholangiocarcinoma by Eliminating Transcriptional Compensation.
    Article Snippet: For pX330-sgP19 (sgp19), pX330 (Addgene #42230) was used as the template, with a single sgp19 duplex integrated. sgRNA sequences for the target genes are listed in Supplementary Table 10. .. AAV8-TBG/Cre and Tamoxifen administration Mice were injected intravenously with 1 × 1012 genome copies (GCs) of adeno-associated virus serotype 8 (AAV8) encoding eGFP or Cre recombinase under the hepatocyte-specific thyroid binding globulin promoter (AAV8-TBG-eGFP; Addgene#105535-AAV8, AAV8-TBG-Cre; Addgene#107787-AAV8) in 100 ul saline. .. CreERT2 recombinase was induced by intraperitoneal tamoxifen injections (20 mg/ml) in corn oil (Thermo Scientific#AC405435000) as previously described elsewhere(5).



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    TaKaRa cre recombinase gesicles
    A, Experimental workflow for generating and assaying prostate organoid morphology. B, TKO organoids are more resistant to an AR degrader. Viability (y axis) of control (NTC, left) and TKO (right) organoids at different concentrations (x axis) of an AR degrader. IC50 values are shown at bottom left. C,D, Cell state heterogeneity in TKO organoids. C, UMAP embeddings of scRNA-seq profiles (dots) of NTC and TKO cells (as in ) colored by gene module scores. D, Fraction of cells (right) with top score for each gene module in control (NTC) and TKO organoids (x axis). Right: Enriched top GO terms (FDR<0.05 as in ) in each module. E, Neoantigen and GFP expression in NINJA prostate organoids. Flow cytometry plots of percentage of cells expressing GFP, in-frame with neoantigens, in NINJA prostate organoids without <t>CRE</t> <t>recombinase</t> (CRE), doxycycline, and tamoxifen (left), with CRE but without doxycycline and tamoxifen (middle), and with CRE, doxycycline and tamoxifen for 72 hours (right). F , Experimental mouse model. TKO organoids from NINJA mice treated with doxycycline and tamoxifen pre-transplantation were transplanted into immunocompetent (C57BL/6, n=10) and immunodeficient (NSG, n=10) mice. G,H CD8 T cell infiltration. G, (Left) Workflow. Right: Representative flow cytometry plots from stained (left) and unstained (right) samples, gated for CD8a, MHC class I tetramer (for neoantigen-specific T cells), and PD1 expression. H, Percentage (y axis) of neoantigen-specific (tetramer-positive, left) and PD1-positive CD8+ T cells (right), at 6- and 12-weeks post-transplantation with castration (x axis). Dots: individual mice. I , Prostates from different experimental groups.
    Cre Recombinase Gesicles, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Laboratory cre recombinase mouse lines
    A, Experimental workflow for generating and assaying prostate organoid morphology. B, TKO organoids are more resistant to an AR degrader. Viability (y axis) of control (NTC, left) and TKO (right) organoids at different concentrations (x axis) of an AR degrader. IC50 values are shown at bottom left. C,D, Cell state heterogeneity in TKO organoids. C, UMAP embeddings of scRNA-seq profiles (dots) of NTC and TKO cells (as in ) colored by gene module scores. D, Fraction of cells (right) with top score for each gene module in control (NTC) and TKO organoids (x axis). Right: Enriched top GO terms (FDR<0.05 as in ) in each module. E, Neoantigen and GFP expression in NINJA prostate organoids. Flow cytometry plots of percentage of cells expressing GFP, in-frame with neoantigens, in NINJA prostate organoids without <t>CRE</t> <t>recombinase</t> (CRE), doxycycline, and tamoxifen (left), with CRE but without doxycycline and tamoxifen (middle), and with CRE, doxycycline and tamoxifen for 72 hours (right). F , Experimental mouse model. TKO organoids from NINJA mice treated with doxycycline and tamoxifen pre-transplantation were transplanted into immunocompetent (C57BL/6, n=10) and immunodeficient (NSG, n=10) mice. G,H CD8 T cell infiltration. G, (Left) Workflow. Right: Representative flow cytometry plots from stained (left) and unstained (right) samples, gated for CD8a, MHC class I tetramer (for neoantigen-specific T cells), and PD1 expression. H, Percentage (y axis) of neoantigen-specific (tetramer-positive, left) and PD1-positive CD8+ T cells (right), at 6- and 12-weeks post-transplantation with castration (x axis). Dots: individual mice. I , Prostates from different experimental groups.
    Cre Recombinase Mouse Lines, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A, Experimental workflow for generating and assaying prostate organoid morphology. B, TKO organoids are more resistant to an AR degrader. Viability (y axis) of control (NTC, left) and TKO (right) organoids at different concentrations (x axis) of an AR degrader. IC50 values are shown at bottom left. C,D, Cell state heterogeneity in TKO organoids. C, UMAP embeddings of scRNA-seq profiles (dots) of NTC and TKO cells (as in ) colored by gene module scores. D, Fraction of cells (right) with top score for each gene module in control (NTC) and TKO organoids (x axis). Right: Enriched top GO terms (FDR<0.05 as in ) in each module. E, Neoantigen and GFP expression in NINJA prostate organoids. Flow cytometry plots of percentage of cells expressing GFP, in-frame with neoantigens, in NINJA prostate organoids without <t>CRE</t> <t>recombinase</t> (CRE), doxycycline, and tamoxifen (left), with CRE but without doxycycline and tamoxifen (middle), and with CRE, doxycycline and tamoxifen for 72 hours (right). F , Experimental mouse model. TKO organoids from NINJA mice treated with doxycycline and tamoxifen pre-transplantation were transplanted into immunocompetent (C57BL/6, n=10) and immunodeficient (NSG, n=10) mice. G,H CD8 T cell infiltration. G, (Left) Workflow. Right: Representative flow cytometry plots from stained (left) and unstained (right) samples, gated for CD8a, MHC class I tetramer (for neoantigen-specific T cells), and PD1 expression. H, Percentage (y axis) of neoantigen-specific (tetramer-positive, left) and PD1-positive CD8+ T cells (right), at 6- and 12-weeks post-transplantation with castration (x axis). Dots: individual mice. I , Prostates from different experimental groups.
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    Cell Signaling Technology Inc primary antibody anti cre recombinase d7l7l xp rabbit mab
    A, Experimental workflow for generating and assaying prostate organoid morphology. B, TKO organoids are more resistant to an AR degrader. Viability (y axis) of control (NTC, left) and TKO (right) organoids at different concentrations (x axis) of an AR degrader. IC50 values are shown at bottom left. C,D, Cell state heterogeneity in TKO organoids. C, UMAP embeddings of scRNA-seq profiles (dots) of NTC and TKO cells (as in ) colored by gene module scores. D, Fraction of cells (right) with top score for each gene module in control (NTC) and TKO organoids (x axis). Right: Enriched top GO terms (FDR<0.05 as in ) in each module. E, Neoantigen and GFP expression in NINJA prostate organoids. Flow cytometry plots of percentage of cells expressing GFP, in-frame with neoantigens, in NINJA prostate organoids without <t>CRE</t> <t>recombinase</t> (CRE), doxycycline, and tamoxifen (left), with CRE but without doxycycline and tamoxifen (middle), and with CRE, doxycycline and tamoxifen for 72 hours (right). F , Experimental mouse model. TKO organoids from NINJA mice treated with doxycycline and tamoxifen pre-transplantation were transplanted into immunocompetent (C57BL/6, n=10) and immunodeficient (NSG, n=10) mice. G,H CD8 T cell infiltration. G, (Left) Workflow. Right: Representative flow cytometry plots from stained (left) and unstained (right) samples, gated for CD8a, MHC class I tetramer (for neoantigen-specific T cells), and PD1 expression. H, Percentage (y axis) of neoantigen-specific (tetramer-positive, left) and PD1-positive CD8+ T cells (right), at 6- and 12-weeks post-transplantation with castration (x axis). Dots: individual mice. I , Prostates from different experimental groups.
    Primary Antibody Anti Cre Recombinase D7l7l Xp Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A, Experimental workflow for generating and assaying prostate organoid morphology. B, TKO organoids are more resistant to an AR degrader. Viability (y axis) of control (NTC, left) and TKO (right) organoids at different concentrations (x axis) of an AR degrader. IC50 values are shown at bottom left. C,D, Cell state heterogeneity in TKO organoids. C, UMAP embeddings of scRNA-seq profiles (dots) of NTC and TKO cells (as in ) colored by gene module scores. D, Fraction of cells (right) with top score for each gene module in control (NTC) and TKO organoids (x axis). Right: Enriched top GO terms (FDR<0.05 as in ) in each module. E, Neoantigen and GFP expression in NINJA prostate organoids. Flow cytometry plots of percentage of cells expressing GFP, in-frame with neoantigens, in NINJA prostate organoids without CRE recombinase (CRE), doxycycline, and tamoxifen (left), with CRE but without doxycycline and tamoxifen (middle), and with CRE, doxycycline and tamoxifen for 72 hours (right). F , Experimental mouse model. TKO organoids from NINJA mice treated with doxycycline and tamoxifen pre-transplantation were transplanted into immunocompetent (C57BL/6, n=10) and immunodeficient (NSG, n=10) mice. G,H CD8 T cell infiltration. G, (Left) Workflow. Right: Representative flow cytometry plots from stained (left) and unstained (right) samples, gated for CD8a, MHC class I tetramer (for neoantigen-specific T cells), and PD1 expression. H, Percentage (y axis) of neoantigen-specific (tetramer-positive, left) and PD1-positive CD8+ T cells (right), at 6- and 12-weeks post-transplantation with castration (x axis). Dots: individual mice. I , Prostates from different experimental groups.

    Journal: bioRxiv

    Article Title: Spatiotemporal profiling reveals the role of inflammatory niche in driving prostate cancer

    doi: 10.64898/2026.04.19.719485

    Figure Lengend Snippet: A, Experimental workflow for generating and assaying prostate organoid morphology. B, TKO organoids are more resistant to an AR degrader. Viability (y axis) of control (NTC, left) and TKO (right) organoids at different concentrations (x axis) of an AR degrader. IC50 values are shown at bottom left. C,D, Cell state heterogeneity in TKO organoids. C, UMAP embeddings of scRNA-seq profiles (dots) of NTC and TKO cells (as in ) colored by gene module scores. D, Fraction of cells (right) with top score for each gene module in control (NTC) and TKO organoids (x axis). Right: Enriched top GO terms (FDR<0.05 as in ) in each module. E, Neoantigen and GFP expression in NINJA prostate organoids. Flow cytometry plots of percentage of cells expressing GFP, in-frame with neoantigens, in NINJA prostate organoids without CRE recombinase (CRE), doxycycline, and tamoxifen (left), with CRE but without doxycycline and tamoxifen (middle), and with CRE, doxycycline and tamoxifen for 72 hours (right). F , Experimental mouse model. TKO organoids from NINJA mice treated with doxycycline and tamoxifen pre-transplantation were transplanted into immunocompetent (C57BL/6, n=10) and immunodeficient (NSG, n=10) mice. G,H CD8 T cell infiltration. G, (Left) Workflow. Right: Representative flow cytometry plots from stained (left) and unstained (right) samples, gated for CD8a, MHC class I tetramer (for neoantigen-specific T cells), and PD1 expression. H, Percentage (y axis) of neoantigen-specific (tetramer-positive, left) and PD1-positive CD8+ T cells (right), at 6- and 12-weeks post-transplantation with castration (x axis). Dots: individual mice. I , Prostates from different experimental groups.

    Article Snippet: For in vitro delivery of CRE recombinase, Takara’s CRE recombinase gesicles (Takara Bio USA, (Catalog No. 631449) were used according to the manufacturer’s instructions.

    Techniques: Control, Expressing, Flow Cytometry, Immunopeptidomics, Transplantation Assay, Staining

    A Cre recombinase‐based dual reporter system distinguishes mixed delivery and transduction. (A) Schematic depicting the expected function of the stoplight dual‐reporter system in which Cre expressed in recipient cells acts upon incoming AAV genomes to switch them from expression of a red reporter to a green reporter. Free AAV mediates transduction only (green); EV‐AAV mediates both mixed delivery (red) and transduction (green) and sometimes both (yellow); No Rep/Cap EVs can only mediate mixed delivery (red). (B) Cre‐responsive plasmids were evaluated in this experiment. (C, D) Mixed delivery (C) and transduction (D) conferred by various vector compositions. Note: some mCherry gene expression could occur in recipient cells prior to Cre‐mediated recombination (or in the possible absence of recombination), such that (C) includes both mixed delivery and this ambiguous de novo gene expression. Samples were normalized to include 1e9 vector genomes per well (for AAV crude lysate conditions) or a volume‐equivalent of the AAV2 crude lysate condition for No Rep/Cap crude lysate conditions (10 4 recipient cells). Experiments were performed in biological triplicate. One of two independent experiments is shown (second experiment: Figure <xref ref-type= S10 ). Error bars indicate the standard error of the mean. Multicomparison statistical analysis was performed using a two‐way ANOVA test, followed by Tukey's multiple comparison test to evaluate specific comparisons (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). NLS, nuclear localization sequence; ns, not significant; PBS, phosphate‐buffered saline. " width="100%" height="100%">

    Journal: Journal of Extracellular Vesicles

    Article Title: Distinguishing Pseudotransduction and True Transduction Enables Characterization and Bioengineering of Extracellular Vesicle‐Adeno‐Associated Virus Vectors

    doi: 10.1002/jev2.70258

    Figure Lengend Snippet: A Cre recombinase‐based dual reporter system distinguishes mixed delivery and transduction. (A) Schematic depicting the expected function of the stoplight dual‐reporter system in which Cre expressed in recipient cells acts upon incoming AAV genomes to switch them from expression of a red reporter to a green reporter. Free AAV mediates transduction only (green); EV‐AAV mediates both mixed delivery (red) and transduction (green) and sometimes both (yellow); No Rep/Cap EVs can only mediate mixed delivery (red). (B) Cre‐responsive plasmids were evaluated in this experiment. (C, D) Mixed delivery (C) and transduction (D) conferred by various vector compositions. Note: some mCherry gene expression could occur in recipient cells prior to Cre‐mediated recombination (or in the possible absence of recombination), such that (C) includes both mixed delivery and this ambiguous de novo gene expression. Samples were normalized to include 1e9 vector genomes per well (for AAV crude lysate conditions) or a volume‐equivalent of the AAV2 crude lysate condition for No Rep/Cap crude lysate conditions (10 4 recipient cells). Experiments were performed in biological triplicate. One of two independent experiments is shown (second experiment: Figure S10 ). Error bars indicate the standard error of the mean. Multicomparison statistical analysis was performed using a two‐way ANOVA test, followed by Tukey's multiple comparison test to evaluate specific comparisons (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). NLS, nuclear localization sequence; ns, not significant; PBS, phosphate‐buffered saline.

    Article Snippet: RepCap plasmids for AAV2 (pRep2Cap2) and AAV6 (pRep2Cap6) were gifts from the Vector Core at the University of Pennsylvania (Penn Vector Core (RRID: SCR_022432)). pcDNA is plasmid pPD005 (Addgene plasmid # 138749; http://n2t.net/addgene:138749 ; RRID:Addgene_138749) (Donahue et al. ). psPAX2 and pMD2.G plasmids were gifted by William Miller from Northwestern University. pHIE822 was created by introducing point mutations (K47Q, R354A) in the VSV‐G‐encoding gene of pMD2.G using site‐directed mutagenesis by PCR. pCMV‐VSV‐G(P127D)‐Myc (pJB042) was a gift from Wesley Sundquist (Addgene plasmid # 80055; http://n2t.net/addgene:80055 ; RRID:Addgene_80055) (Votteler et al. ). pHIE963 was created using standard restriction enzyme cloning to insert a Cre recombinase gene into a third‐generation lentiviral transfer vector provided by Twist Bioscience (pTwist Lenti SFFV PuroR).

    Techniques: Transduction, Expressing, Plasmid Preparation, Gene Expression, Comparison, Sequencing, Saline

    Generation and validation of club cell-specific AHR knockout mice ( Ahr ΔCC). (a) Schematic of breeding strategy to generate Ahr ΔCC mice by crossing Ahr fl/fl mice with Scgb1a1-CreER TM mice, followed by tamoxifen induction. (b) Immunofluorescence staining showing club cell marker CC10 (green), AHR (red) and DAPI (blue) in lung sections of Cre-negative Ahr fl/fl LM control (top panel) and Ahr ΔCC (bottom panel) mice. (c) Representative flow cytometry plots of lung epithelial cells gated as CD45 − CD31 − EpCAM + CC10 + cells isolated from lungs of LM (left) or Ahr ΔCC (center) mice and Fluorescence-minus-one (FMO) control for AHR staining (right). (d) Quantification of the percentage of AHR + CC10 + cells in the lungs of LM (white bar) and Ahr ΔCC (gray bar) mice. Data represent mean ± SEM, n = 3-4 mice per group. Statistical significance was determined using Student's t-test; ∗p < 0.05.

    Journal: Redox Biology

    Article Title: Aryl hydrocarbon receptor in club cells drives Th17-mediated lung injury following inhalation exposure to environmentally persistent free radicals

    doi: 10.1016/j.redox.2026.104105

    Figure Lengend Snippet: Generation and validation of club cell-specific AHR knockout mice ( Ahr ΔCC). (a) Schematic of breeding strategy to generate Ahr ΔCC mice by crossing Ahr fl/fl mice with Scgb1a1-CreER TM mice, followed by tamoxifen induction. (b) Immunofluorescence staining showing club cell marker CC10 (green), AHR (red) and DAPI (blue) in lung sections of Cre-negative Ahr fl/fl LM control (top panel) and Ahr ΔCC (bottom panel) mice. (c) Representative flow cytometry plots of lung epithelial cells gated as CD45 − CD31 − EpCAM + CC10 + cells isolated from lungs of LM (left) or Ahr ΔCC (center) mice and Fluorescence-minus-one (FMO) control for AHR staining (right). (d) Quantification of the percentage of AHR + CC10 + cells in the lungs of LM (white bar) and Ahr ΔCC (gray bar) mice. Data represent mean ± SEM, n = 3-4 mice per group. Statistical significance was determined using Student's t-test; ∗p < 0.05.

    Article Snippet: Male Ahr tm3.1Bra /J mice carrying a floxed exon 2 allele of the Ahr gene (JAX stock #006203) and female B6N.129S6(Cg)- Scgb1a1 tm1(cre/ERT)Blh /J mice expressing tamoxifen-inducible Cre recombinase under the control of the club cell-specific Scgb1a1 promoter (JAX stock #016225) were obtained from Jackson Laboratory.

    Techniques: Biomarker Discovery, Knock-Out, Immunofluorescence, Staining, Marker, Control, Flow Cytometry, Isolation, Fluorescence

    A, Experimental workflow for generating and assaying prostate organoid morphology. B, TKO organoids are more resistant to an AR degrader. Viability (y axis) of control (NTC, left) and TKO (right) organoids at different concentrations (x axis) of an AR degrader. IC50 values are shown at bottom left. C,D, Cell state heterogeneity in TKO organoids. C, UMAP embeddings of scRNA-seq profiles (dots) of NTC and TKO cells (as in ) colored by gene module scores. D, Fraction of cells (right) with top score for each gene module in control (NTC) and TKO organoids (x axis). Right: Enriched top GO terms (FDR<0.05 as in ) in each module. E, Neoantigen and GFP expression in NINJA prostate organoids. Flow cytometry plots of percentage of cells expressing GFP, in-frame with neoantigens, in NINJA prostate organoids without CRE recombinase (CRE), doxycycline, and tamoxifen (left), with CRE but without doxycycline and tamoxifen (middle), and with CRE, doxycycline and tamoxifen for 72 hours (right). F , Experimental mouse model. TKO organoids from NINJA mice treated with doxycycline and tamoxifen pre-transplantation were transplanted into immunocompetent (C57BL/6, n=10) and immunodeficient (NSG, n=10) mice. G,H CD8 T cell infiltration. G, (Left) Workflow. Right: Representative flow cytometry plots from stained (left) and unstained (right) samples, gated for CD8a, MHC class I tetramer (for neoantigen-specific T cells), and PD1 expression. H, Percentage (y axis) of neoantigen-specific (tetramer-positive, left) and PD1-positive CD8+ T cells (right), at 6- and 12-weeks post-transplantation with castration (x axis). Dots: individual mice. I , Prostates from different experimental groups.

    Journal: bioRxiv

    Article Title: Spatiotemporal profiling reveals the role of inflammatory niche in driving prostate cancer

    doi: 10.64898/2026.04.19.719485

    Figure Lengend Snippet: A, Experimental workflow for generating and assaying prostate organoid morphology. B, TKO organoids are more resistant to an AR degrader. Viability (y axis) of control (NTC, left) and TKO (right) organoids at different concentrations (x axis) of an AR degrader. IC50 values are shown at bottom left. C,D, Cell state heterogeneity in TKO organoids. C, UMAP embeddings of scRNA-seq profiles (dots) of NTC and TKO cells (as in ) colored by gene module scores. D, Fraction of cells (right) with top score for each gene module in control (NTC) and TKO organoids (x axis). Right: Enriched top GO terms (FDR<0.05 as in ) in each module. E, Neoantigen and GFP expression in NINJA prostate organoids. Flow cytometry plots of percentage of cells expressing GFP, in-frame with neoantigens, in NINJA prostate organoids without CRE recombinase (CRE), doxycycline, and tamoxifen (left), with CRE but without doxycycline and tamoxifen (middle), and with CRE, doxycycline and tamoxifen for 72 hours (right). F , Experimental mouse model. TKO organoids from NINJA mice treated with doxycycline and tamoxifen pre-transplantation were transplanted into immunocompetent (C57BL/6, n=10) and immunodeficient (NSG, n=10) mice. G,H CD8 T cell infiltration. G, (Left) Workflow. Right: Representative flow cytometry plots from stained (left) and unstained (right) samples, gated for CD8a, MHC class I tetramer (for neoantigen-specific T cells), and PD1 expression. H, Percentage (y axis) of neoantigen-specific (tetramer-positive, left) and PD1-positive CD8+ T cells (right), at 6- and 12-weeks post-transplantation with castration (x axis). Dots: individual mice. I , Prostates from different experimental groups.

    Article Snippet: For in vitro delivery of CRE recombinase, Takara’s CRE recombinase gesicles (Takara Bio USA, (Catalog No. 631449) were used according to the manufacturer’s instructions.

    Techniques: Control, Expressing, Flow Cytometry, Immunopeptidomics, Transplantation Assay, Staining